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Fig. 7. Effects of interfering with BMP signalling on the distribution of
transcripts known to be involved in NR and RPE development. (A-D)
Control experiments overexpressing a viral RCAS (B) (labelled RCAS-B)
construct only (parallel sections). (A) Eye morphology is normal following
viral infection with RCAS (B). Expression of the viral reverse
transcriptase gene (RT) indicates infected areas of the RPE
(arrows). (B) In the eyes infected with RCAS (B), Otx2 expression is
still restricted to the RPE (arrowheads) and no transcripts are observed in
the NR at stage 20. (C) Following viral injection of RCAS (B), MMP115
expression is unchanged and is restricted to the RPE (arrowheads). (D)
Expression of the NR-specific marker Chx10 is not observed in the
outer layer of the optic cup following injection of RCAS (B) (arrowheads).
(E-H) The effects following injection of the viral dnBmpR1b-RCAS (B)
construct at stage 6. (E) Following injection of dnBmpR1b, a large region of
the outer layer of the optic cup is thickened (arrowheads). The infection of
the RPE is shown by the expression of RT. (F) Otx2
expression weakens in the outer layer of the optic cup following injection of
dnBmpR1b (arrowheads). (G) MMP115 expression is downregulated
following inhibition of BMP signalling (arrowheads). (H) Expression of the
NR-specific gene Chx10 is induced in the outer layer of the optic cup
following viral overexpression of the dnBmpR1b construct (arrowheads).
(I) RT expression in the outer layer of the optic cup
(arrowheads) following viral infection of the dnBmpR1b construct at stage 7.
(J) MMP115 expression is downregulated (arrowheads) in the
infected region of the outer layer of the optic cup. Strong MMP115
expression is still observed in the single-layered portion (arrow). (K)
RT expression within the proximal RPE (arrowheads) following
infection at stage 10. (L) The arrowheads indicate the expression of
the NR marker gene Chx10 in the RPE in the parallel section. L, lens;
NR, neural retina.
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