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Fig. 8. rga-3/4(RNAi) causes strong gonad defects in
let-502(sb106) mutants. (A) Gonad arms were extracted from
worms and stained with rhodamin-phalloidin and DAPI. The gonads are oriented
with the distal tip pointing towards the left. A schematic drawing of the
gonad is included. The arrowheads point to the rachis formation defects. The
arrow points to the `hole' in the let-502(sb106) rga-3/4(RNAi) gonad.
(B) A gonad from the let-502(ok1283) mutant, which represents
a knock-out allele, was stained as in A. The arrow points to the `hole'.
(C) Quantification of gonad defects of N2 and let-502(sb106)
animals treated with rga-3/4 or cyk-4 dsRNA. The number of
gonads used for quantification of a particular phenotype is given in
parenthesis. Depending on how the gonads adhered to the glass surface, not all
phenotypes could be quantified in the same gonad. Ambiguous-looking gonads
were not included in the analysis. Scale bar: 50 µm.
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