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Figure 3


Fig. 3. Temporal dependency of early gastrula-stage A-neural markers to Nodal signal inhibition. Markers analysed are indicated on the left of the graphs. Graphs show the percentage of embryos with strong or weak expression in the lateral neural precursors (marked with arrowheads). Scoring was as follows: Ci-Snail, strong = at least two cells positive, weak = one cell positive or very weak expression in one to four cells; Ci-Ngn, strong = at least one cell positive, weak = at least one blastomere showing very weak expression; cicl007j15, strong = at least two lateral cells positive, weak = one lateral cell positive or weak expression in one to four lateral cells. For cicl007j15, only embryos with expression in one to four medial cells were included in the analysis. Numbers shown above the bars indicates the total number of embryos analysed. On the x-axis is shown the developmental time point when embryos were placed in inhibitor: C, control; 16, 32, 64, 76, 110 = cell stage.





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