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Fig. 3. Temporal dependency of early gastrula-stage A-neural markers to Nodal
signal inhibition. Markers analysed are indicated on the left of the
graphs. Graphs show the percentage of embryos with strong or weak expression
in the lateral neural precursors (marked with arrowheads). Scoring was as
follows: Ci-Snail, strong = at least two cells positive, weak = one
cell positive or very weak expression in one to four cells; Ci-Ngn,
strong = at least one cell positive, weak = at least one blastomere showing
very weak expression; cicl007j15, strong = at least two lateral cells
positive, weak = one lateral cell positive or weak expression in one to four
lateral cells. For cicl007j15, only embryos with expression in one to
four medial cells were included in the analysis. Numbers shown above the bars
indicates the total number of embryos analysed. On the x-axis is
shown the developmental time point when embryos were placed in inhibitor: C,
control; 16, 32, 64, 76, 110 = cell stage.