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Fig. 4. Expression of A-line neural markers at the early gastrula stage
following Delta2/Notch inhibition or ectopic activation. (A)
Expression of Ci-Delta2 at the 64- and 76-cell stages. Embryos and
schematics are shown in lateral view with the vegetal pole to the left.
Ci-Delta2-expressing blastomeres surround A7.8, the founder cell of
columns 3 and 4, at the 64-cell stage, and surround A8.16, the precursor cell
of column 4, at the 76-cell stage. Blastomere names are indicated. (B)
Expression of markers, indicated on the left, at the early gastrula stage
following the treatments indicated above the panels. For lateral markers,
numbers indicate any expression seen in A-line neural cells over the total
number of embryos analysed. The inserts for Ci-Delta2 expression are
lateral views of embryos, showing that expression is slightly but consistently
upregulated following Delta2/Notch inhibition, particularly in b-line cells.
For cicl007j15, numbers indicate the mean number of positive cells
(c) per embryo; n, total number of embryos analysed. Similar results were
observed with dnDel2 injection (Ci-Snail, 35/35; Ci-Ngn,
5/48; cicl007j15, 3.2c; n=24). (C) Expression of
markers, shown above the panels, following FOG::Delta2
electroporation. Numbers indicate: for Ci-Snail, the number of
embryos showing expression represented by the panel over the total number of
embryos analysed; for Ci-Hesb and Ci-Ngn, the number of
embryos showing ectopic expression in A-line neural cells over the total
number of embryos analysed. Ectopic expression of Ci-Hesb and
Ci-Ngn was also sometimes observed in additional vegetal cells (data
not shown). The insert on the bottom right of Ci-Hesb panel shows
expression of Ci-Hesb in a control embryo.
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