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Fig. 5. Expression of various neural plate markers following Delta2/Notch
inhibition or ectopic expression. The marker analysed is indicated on the
left and embryo treatment is indicated above the panels. Graphs show the
percentage of embryos with ectopic, strong or weak expression according to the
key indicated below. The same key is used for all subsequent figures. For
Ci-Hesb and Ci-Chordin, `weak' means reduced expression
compared with the majority of controls. For the remaining markers, `strong'
means at least one A-line neural precursor cell with strong expression and
`weak' means at least one cell with downregulated expression. Occasionally,
following disruption of Delta2/Notch signalling, ectopic expression was seen
for Ci-FGF8/17/18 and Ci-ephrinAb. This ectopic expression
appeared in column 4. Following FOG::Delta2 electroporation
(abbreviated to FOG::D2), Ci-FGF8/17/18 exhibited frequent unilateral
expression. Of those scored positive, 49% expressed Ci-FGF8/17/18 on
one side only (compared with 9% of control embryos from the same batches).
Ectopic expression of Ci-COE following FOG::Delta2
electroporation appeared in column 3 (A9.30) in the majority of cases (see
text for details) with 54% of these cases exhibiting unilateral A9.30
expression and 46% bilateral. Ectopic expression of Ci-MRF was
observed only in column 3 (A9.29). Of those exhibiting ectopic expression, 66%
had column 3 (A9.29) expression on one side only. In most cases, column 3
(A9.29) expression of Ci-MRF appeared slightly weaker than the
expression in column 4 (A9.31). For Ci-FoxB, expression in both
control and treated embryos was frequently (around half of those scored
positive) observed on one side only. Arrowheads point to ectopic expression on
the panels.
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