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Fig. 8. Schematic of de novo shoot meristem organization from
callus. Auxin-rich CIM (A) induces proliferation of multipotent
cells in the root leading to callus formation (B). (C) Transfer
to cytokinin-rich SIM induces partition of cell identity and behavior within
callus marked by the CUC2 (yellow) and WUS (red) reporters.
Arrowhead indicates shoot progenitors. (D) Clusters of
CUC2-labeled shoot progenitors proliferate among neighboring
WUS expressing (red lines) non-progenitor cells in areas of high
cytokinin and low auxin response. (E) 24-48 hours later, PIN1
and ML1 reporters (both green) are upregulated within the superficial
layer of the shoot promeristem while STM (blue) is upregulated in a
ring of surrounding cells and within the promeristem. Within the membrane of
shoot progenitors, PIN1 protein is directed towards the apex of the
promeristem (arrows), and thus is predicted to transport auxin into the
promeristem from surrounding cells. (F) 48-96 hours later,
PIN1 becomes locally upregulated within the peripheral zone and marks
sites of primordial initiation. PIN1 protein becomes locally polarized towards
sites of primordia formation (arrows). FIL (magenta) is expressed in
the abaxial sides of newly initiated primordia. CLV3 expression
(teal) is initiated within the central zone after WUS expression
(red) initiates within the center of the meristem. pSTM::STM-VENUS is
expressed within the meristem.
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