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Fig. 4. cNanog expression during chick embryo development.
(A-J) Whole-mount in situ hybridisation to cNanog transcripts.
Nanog transcripts are localised in the epiblast of the area pellucida
and area opaca of the pre-streak embryo (A, stage XI; B, stage XII), but not
in the hypoblast (B'). From stage XIV, cNanog mRNA disappears
from the posterior area pellucida (C,C') and from the growing primitive
streak (D, stage 3; E,E', stage 3+; F, stage 4+). cNanog
transcripts are downregulated in the epiblast from stage 4+ (F), and are
confined anteriorly in a crescent region in the epiblast (G, stage 5+). At
stage 6 (H), expression is restricted to the neural plate and the neural tube
(I, stage 7; J, stage 8). cNanog is also expressed in scattered cells
in the germinal crescent from stage 4 (arrowhead in I and H'). B'
is a longitudinal section of the embryo in B, anterior at the right;
C',E',H' are transverse sections of embryos in C,E,H,
respectively. ep, epiblast; gd, gonad; ms, mesonephros; np, neural plate; nt,
neural tube; ps, primitive streak.
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