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Fig. 5. cPouV and cNanog are expressed in germ cells during
later embryonic development. (A-H) At stage 33, cPouV mRNA
is detected in the developing gonad, which is attached to the mesonephros
(A,B), in the germ cells (D), as detected by co-localisation (F) of
cPouV (D) both with Cvh (chicken Vasa, E)
expression and with SSEA-1-positive cells (G) revealed on adjacent sections
counterstained by Hoechst and cPouV probe (H, arrows). (B) Section of
gonad shown in A. (C) Bright field of the stage 33 gonad used for in situ
hybridisation (D,E,F). (I-M) At stage 33, cNanog is highly
expressed in gonads and in mesonophros tubules (I) and gonad (I,J), especially
in germ cells (K), as revealed by SSEA-1 staining (L) on the same cells that
express cNanog in adjacent sections (M, arrow) counterstained by Hoechst (L).
(K) Section of dissected gonad from the urogenital tract (J). ms, mesonephros;
gd, gonads. Scale bars: 15 µm.
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