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Figure 2


Fig. 2. Oskimm dominantly influences polar granule and nuclear body morphology. Pole cells displaying GFP-Aub (green) and immunostained for Osk (red) from cellular blastoderm stage (A,D) or gastrulation stage (B,C,E,F) embryos, with enlarged views of nuclear bodies in C and F. Embryos are from otherwise wild-type mothers bearing a single copy of the following transgenes: A-C, P[osk+]; D-F, P[oskimm]. P[osk+] polar granules are spherical (A,B). In the presence of both endogenous Osk and Oskimm (D,E), the polar granules fuse into one to three large aggregates. Nuclear bodies are normally spherical (C), but in P[oskimm] embryos (F) the nuclear bodies show deformations and discontinuities (arrows). Scale bars: A,B,D,E, 5 µm; C,F, 2 µm.





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