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Fig. 2. sad-1 and nab-1 mutants fail to restrict axonal fate
in VD neurons. (A) GABAergic synapses along the dorsal cord in
wild-type, sad-1- and nab-1-mutant young adults visualized
by juIs1 marker. nab-1 mutants show no synaptic morphology
defects. After unc-30 RNAi treatment to block juIs1
expression in DD neurons, ectopic synaptic-vesicle clusters in VD neurons were
detected in both nab-1 and sad-1 mutants. Arrowhead shows
the dorsal nerve cord. (B) nab-1 mutants have a reduced number
of synapses along the axon of VD neurons before and after unc-30 RNAi
treatment. Arrow shows VD neuron cell body. (C) nab-1 and
sad-1 mutants display ectopic dorsal SYD-2::GFP, an active-zone
marker, in VD neurons. Arrowhead shows the dorsal nerve cord. (D)
juIs1 phenotypes in wild-type (wt, left upper panels) or
nab-1 (left lower panels) animals were analyzed with MathLab software
(developed by C. Mok, University of Toronto, Canada). The intensity and width
of individual fluorescent punctum, as well as the distance between puncta
(inter-punctal width), was calculated from juIs1 images of wild-type
and nab-1 animals. Left panels; a graphical representation and the
corresponding juIs1 image. Average values of the punctal intensity
(upper right panel), punctal width (lower right panel) and interpunctal width
(lower right panel) were plotted and shown. No significant difference was
found between wild-type and nab-1 values (n=13,
P>0.05). Scale bar: 5 µm in A,C.
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