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Fig. 1. (A) Generation of floxed/deleted Gata3 alleles. LoxP
sites were introduced in the start codon (in exon 2) and in the second intron
(blue flags) of Gata3. A Puro cassette, flanked by FRT sites
(pink flags) and coupled to an nlsLacZ gene lacking a polyA signal
was introduced in intron 2. Arrows indicate lengths of
NotI-SalI restriction enzyme fragments that hybridize to the
external 5' probe. A Polyoma thymidine kinase gene (PyTK) was
added 5' to the construct to counter select against random integration.
Flp recombinase mice (Buchholz et al.,
1998) were used to remove the Puro cassette. After
recombination by Cre recombinase, resulting in excision of the part of exon 2
that is 3' to the start ATG of Gata3 and the part of intron 2
that is 5' to the EcoRI site, the nlsLacZ reporter
replaces the 3' end of the truncated Gata3 gene. Arrows below
genes indicate direction of transcription. Black boxes correspond to exons. B,
BamHI; C, ClaI; H, HindIII; N, NcoI; R,
EcoRI; S, SacI. (B-T). Phenotypes caused by
homozygous deletion of Gata3 in the skin (ko) versus wt. (B) Delayed
hair growth in K14-Gata3-/- at P10. (C) Short stubby hairs
instead of normal coat of fur. (D) Weight difference in the first months of
life. (E,F) Eyes are still closed, whiskers are present but rudimentary, most
facial hairs have disappeared and remaining hairs are still very short. (G)
After 3 months, most K14-Gata3-/- mice are completely
bald; eyes are still closed. (H,I) The absence of visible nipples in female
K14-Gata3-/- mice. (J,K) The complete loss of hairs from
the abdominal area at 2 months. (L,M) Baldness starts at the head and progress
from anterior to posterior. (N-P) Coat of fur at P26. d, dorsal; v, ventral.
(N) wt back hair; (O) K14-Gata3-/- back hair; (P)
K14-Gata3-/- belly hair (and flakes; note the encircled,
thick rounded tip of the hair instead of the normal fine hair tips). (Q,R)
Scanning electron microscopy of dorsal wt (Q) and
K14-Gata3-/- (R) hairs of 12-week-old mice. Note thickness
of K14-Gata3-/- hair as well as absence of regular cuticle
cells and presence of irregular flakes. (S,T) Around P24,
K14-Gata3-/- skin starts flaking. Scale bar in Q: 50 µm
for Q,R.
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