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Fig. 5. Ascl1-expressing cells rapidly exit the cell cycle and move laterally out of the ventricular zone. Immunofluorescence with antibodies to YFP (green) and the cell cycle marker Ki67 (red) on neural tube sections from Ascl1-CreER^TM;R26R-YFP transgenic embryos with either Ascl1 wild-type (A,A') or Ascl1 null (B,B'), or Nestin-CreER^T2;R26R-YFP transgenic embryos (C,C'). Tamoxifen induction was initiated at E10.5 and expression was examined 24 hours later at E11.5. A'-C' are higher magnification images of comparable regions boxed in A-C. (A,A') YFP labeled cells in Ascl1-CreER^TM;R26R-YFP do not co-label with Ki67. (B,B') In the absence of Ascl1, YFP and Ki67 co-labeled cells are found in the ventricular zone (B', arrowheads). (C,C') By contrast, some YFP labeled cells in Nestin-CreER^T2;R26R-YFP co-label with Ki67 and are found in the ventricular zone (C', arrowheads).

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