|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Perichondrial architecture and bone collar development are affected in
prx1cre;Ift88fl/n conditional mutant mice.
(A-D) H&E-stained sections from E18.5 wild-type (A,C) and
conditional mutant (B,D) tibiae. The outer and inner edges of the
perichondrium are marked by green lines. The perichondrium in wild-type
samples (A) is a compact layer flanking the bone anlagen. By contrast, the
cells adjacent to the diaphysis in conditional mutant samples (B) are
disorganized. The perichondrium varies in thickness along the length of the
bone and is flanked by cells that more closely resemble chondrocytes than
osteoblasts (green arrowheads in B). Metaphysis and perichondrium of tibia
sections stained with H&E from E18.5 wild-type (C) and
prx1cre;Ift88fl/n conditional mutant (D) tibiae, show loss
of bone collar formation in conditional mutants. (E,F) Sections
of wild-type (E) and conditional mutant (F) tibiae at E18.5 stained for
alkaline phosphatase activity (blue) to identify the bone collar (arrow).
Sections were counterstained with Nuclear Fast Red. Alkaline phosphatase
expression is visible in the cells of the bone collar flanking the
proliferating and prehypertrophic chondrocytes in wild-type samples (E), but
is absent in the perichondrial region of conditional mutants (F). Insets in E
and F show higher magnification views of the regions indicated by the arrows.
The distal end of the tibia is left in all panels. HC, hypertrophic
chondrocytes.
|
