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Fig. 8. Chondrocytes develop in the perichondrium of
prx1cre;Ift88fl/n conditional mutant tibiae.
(A,B) In sections of wild-type E18.5 tibia (A), cartilage
matrix, identified with Safranin O staining (red), is only seen in the growth
region of the long bone, whereas bone, identified with Fast Green staining
(blue-green), is present along the diaphysis. In conditional mutants (B),
regions of cartilage matrix are evident along the diaphysis of the tibia
(arrowheads) and appear to be derived from the overlying perichondrium.
(C,D) In sections of the wild-type tibia (C) at E18.5, aggrecan
is localized to the hypertrophic region and excluded from the perichondrium by
immunofluorescence. Aggrecan expression is found ectopically in the
perichondrium of the conditional mutant tibia (asterisk in D). Nuclei are
stained blue and the epiphysis is up in both panels. (E) An adjacent
section of the conditional mutant tibia shown in D was stained with Safranin O
and Fast Green to confirm the presence of cartilage matrix in the
perichondrium of the mutant sample.
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