|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Early morphological changes and reduced cell proliferation in the
Lmx1b-/- embryo. (A,B) Nissl-stained
sagittal sections of embryos at E10.5, showing the reduced rhombomere 1 (r1)
and less conspicuous isthmus in the mutant brain (B, arrow), as compared with
the control brain (A, arrow). (C,D) Immunostaining of Ki67,
showing a reduced number of proliferating cells in the tectum and r1 of
Lmx1b-/- embryo (D) as compared with that in the
control (C) at E10.5. (E-H) Sagittal sections of embryos at E12.5,
showing the loss of the constriction between the midbrain and hindbrain (F,
arrowhead) and reduced Math1 expression (E,F, arrows) in the
presumptive cerebellum (H, arrow) in the mutant embryo, as compared with
control brains (E,G). (I,J) Expression domain of Otx2
(arrow) did not show a caudal extension in Lmx1b-/- mutant
embryos (J) as compared with wild-type (I) at E12.5. (K,L)
Transverse sections of embryos at E12.5, showing reduction of Nurr1
expression in the ventral midbrain of the mutant embryo (L) as compared with
the control (K). (M-P) Transverse sections of embryos at E15.5, showing
the loss of the cerebellar vermis (N, arrow) and normal Brn3a
expression in the presumptive red nucleus (P, arrow) in the mutant embryos as
compared with control brains (M,O). (Q,R) Transverse sections of
embryo at E15.5, showing the loss of tyrosine hydroxylase (TH) immunostaining
in the tegmentum of the mutant (R) as compared with the wild-type embryo (Q).
Aq, aqueduct; Cb, cerebellum. Scale bars: A-D, 1000 µm; E-L, 700 µm;
M-R, 500 µm.
|
