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Fig. 4. Analysis of Fgf8, Wnt1, En1 and Pax2
expression in the MHB of Lmx1b-/- embryos.
(A-D) Whole-mount in situ hybridization showing the absence of
Fgf8 expression in the MHB at the 4-somite stage (B, arrow) and in
the isthmus at E9.5 (D, arrow) in the Lmx1b-/-
embryo. Note that Fgf8 expression in other areas of the mutant embryo
(B, arrowhead) was normal as compared with that in the wild-type embryo (A,
arrowhead). (E-H) Lmx1b deletion resulted in reduced
Wnt1 expression in the MHB at the 3-somite stage (F, arrow) and the
loss of Wnt1 expression in the isthmus at E9.5 (H, arrow) as compared
with that in the wild-type brain (E,G). Note that Wnt1 expression in
the roof plate was maintained in the mutant brain at E9.5 (H). (I-L)
Lmx1b deletion caused a marked reduction in En1 expression
in the MHB at the 3-somite stage (J, arrow) and the loss of En1
expression at E9.5 (L, arrow), as compared with that in the control (I,K).
(M-P) Lateral view of embryos showing similar changes in Pax2
expression (arrows) to those found for En1 (I-K).
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