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Fig. 4. Consequences of asymmetric dally expression in the wing.
All wing discs in this figure were stained and imaged together and can
therefore be directly compared. (A-A'') Control ci>GFP
wing disc stained for GFP, P-Mad and Nubbin (Nub, a marker for the
appendage-forming region of the disc). The white and red arrows indicate
expression of P-Mad in the anterior and posterior compartments, respectively.
(B,B') When UAS-dallyRNAi is expressed with
en-Gal4, the extent of P-Mad staining in the P compartment (red
arrow) is reduced as compared with control wing discs (A'). (C)
Driving UAS-dallyRNAi in the P compartment reduces the P:A ratio
(0.65 compared with 1.04 in control discs). (D,E) Driving
UAS-dallyRNAi with en-Gal4 decreases the overall size of the
adult wing by 19% as compared with control (en-Gal4;
UAS-GFP) wings. The decreased P:A ratio is evident in the posterior
shift of vein L4 of en>dallyRNAi wings (magenta arrowhead)
compared with control wings (black arrowhead). (F,F')
Driving UAS-dallyRNAi in the A compartment with ci-Gal4
increases the extent of P-Mad staining in the P compartment (red arrow)
compared with control wing discs (A'). The levels of P-Mad staining in
the AP organizers of these discs is increased compared with control wing discs
(A'; see main text). (G) Driving UAS-dallyRNAi in the A
compartment increases the P:A ratio (1.36 compared with 0.94 in control
discs). (H,I) Although the increased P:A ratio is evident in the
anterior shifting of vein L4 (H, arrowheads), the overall size of ci-Gal4
UAS UAS-dallyRNAi wings is the same as in control wings (I). A, anterior
compartment; P, posterior compartment.
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