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Fig. 2. ednras are expressed in the pharyngeal arches. Whole-mount
RNA in situ hybridizations in wild-type embryos; lateral views with anterior
to the left (A-E,G,H,J,K). Transverse
cryosections through the arches (F,I,L). (A) At 14 hpf,
ednra1 is expressed in cranial NC. (B) At 16 hpf ednra1
marks three streams of cranial NC as well as NC in the trunk (18 hpf, C). (D)
By 24 hpf, ednra1 is expressed throughout the entire DV extent of
every arch. (E) Expression in NC cells is excluded from the endodermal pouches
but is present in cranial ganglia (32 hpf, arrowheads in D,E). (F) Transverse
cryosection at 32 hpf showing that ednra1 is expressed in both the NC
and arch ectoderm. (G) ednra2 expression in cranial vasculature at 20
hpf (arrowheads in G,H,J). (H) ednra2 expression in NC cells of the
arches at 24 hpf. (I) Co-labeling for dlx2a (red) and ednra2
(blue) showing both genes are expressed in NC cells throughout the arch and
excluded from the ectoderm at 28 hpf. (J) At 32 hpf, ednra2 is
expressed in NC, but like ednra1, is excluded from the pharyngeal
endoderm. (K) ednra2 expression in trunk vasculature at 24 hpf. (L)
dlx2a is expressed in NC cells throughout the arch but not the
ectoderm at 28 hpf.
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