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Fig. 1. stripeA and stripeB mRNAs show distinct expression
profiles during embryonic development. Upper panel: schematic
representation of the coding sequences of StripeA and StripeB isoforms, and
the genomic region of the stripe gene (non-coding exons are shown in
black and coding exons in white). Lower panel: whole embryo staining of stage
14 embryo, before muscle-tendon interaction (A-D), stage 15
(E-H), where part of the muscles are attached, or stage 17 embryos,
where muscle-tendon interaction has been established (I-L). Staining
was with anti-StripeA (red, A,E,I); anti-Stripe (blue, B,F,J; an antibody
recognizing both Stripe isoforms); and anti-Myosin heavy chain (MHC, green,
C,G,K). D,H and L are the corresponding merged images. Arrowheads in I point
to the three muscle-bound tendon cells expressing high StripeA levels. The
inset in L represents high magnification of the bracket showing about 14
blue-labeled tendon precursor cells, from which only three cells that are in
close proximity with the three lateral transverse muscles express StripeA.
NLS, nuclear localization signal; ZF, zinc finger.
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