QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. How(S) is required for stripeA elevation. (A-H) Wild-type embryo (A,C,E,G) or how^stru mutant embryo (B,D,F,H) at early stage 16 stained for anti-StripeA (red, A,B) and anti-Stripe (blue, C,D). C and D are the corresponding merged images of A and B. Embryos stained for anti-Shot (red, E,F) and anti-Stripe (blue, G,H). G and H are the corresponding merged images of E and F. Tendon cells of the ventral longitudinal muscles are shown. A significant reduction of StripeA and Shot is detected in the how mutant embryos. (I-K) Embryos carrying either the 69B-gal4 driver alone (I), together with How(L) (J) or together with How(S) (K) stained with anti-StripeA antibody. Tendons of the ventral longitudinal muscles are shown. (L) RT-PCR performed on RNA extracted from how^stru mutant embryos (selected by their negative GFP staining) or wild-type embryos at the age of 14-16 hours AEL, using primers specific for either stripeA (left panel) or stripeB (right panel). The total RNA of each sample was normalized against tubulin levels. (M) RT-PCR with stripeA-(left panel) or stripeB-(right panel) specific primers, performed on RNA extracted from embryos overexpressing How(S) using the stripe-gal4 driver. The embryos were at 14-16 hours AEL.

Return to article