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Fig. 8. stripeA mRNA levels are specifically reduced in crn
mutant embryos. (A) RT-PCR with stripeA- and
stripeB-specific primers, performed on RNA extracted from
crn homozygous mutant or from wild-type embryos at 14-16 hours AEL.
The mRNA levels were normalized against tubulin mRNA levels in each
sample. A specific reduction of stripeA is detected. (B-E)
Stage 16 wild-type (B) or crn mutant (D) embryos stained for Shot
(red) and Stripe (Blue, C,D); C and D are the corresponding merged images. A
significant reduction of Shot is detected in crn mutant embryos.
(F-I) Stage 16 wild-type (F) or crn mutant (H) embryos stained
for How (red) and Stripe (blue, G,I); G and I are the corresponding merged
images. Note that there is no change in How levels in crn mutants.
Arrowheads mark the tendon cells of the ventral longitudinal muscles.
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