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Fig. 1. Molecular profile of the hemangioblast and relationship to other stem
cells. (A) The 37 genes representing the GO term `regulation of
transcription, DNA-dependent' were extracted from the list of 667 genes that
were differentially expressed between BL-CFC and Blast and used to cluster the
duplicate Blast and BL-CFC chips. The scale for relative enrichment of each
transcript is depicted at the bottom: 3-fold or greater enrichment for a
transcript on one chip relative to the other chips is depicted in dark red,
whereas 3-fold less expression of that transcript is in dark blue. (B)
The five GO terms with the highest relative representation in the BL-CFC
profile are graphed with respect to their fractional representation among the
genes of hematopoietic stem cells and their progeny, mature bone marrow cells,
and also relative to the Blast profile. The mostrepresented GO terms in the
progenitor populations relate to nuclear functions, including synthesis of and
binding to nucleic acids. (C) Expression profiles (i.e. lists of genes
enriched in a given cell population) from three previous analyses of
progenitor and progeny gene expression were used to analyze the
progenitor-progeny relationship between BL-CFC and Blast cells. Expression
profiles were clustered based on differences between the overall
representation of GO terms within each profile. Differences between each
profile are represented by Pearson correlation, where 0.0 indicates identical
GO term distribution between two profiles, 1.0 indicates no correlation, and
2.0 indicates negative correlation. Cell profiles are as follows: HSC (1),
Marrow (1), Neural_SC (1), and HSC_EST(1) indicate Hematopoietic Stem Cell,
Bone Marrow Cell, Neural Stem Cell, and Expressed Sequence Tag in
Hematopoietic Stem Cells respectively, from Ivanova et al.
(Ivanova et al., 2002) and
HSC_EST from Phillips et al. (Phillips et
al., 2000). HSC (2), Marrow (2), Neural_SC (2), and Brain (2)
indicate Hematopoietic Stem Cell, Bone Marrow Cell, Neural Stem Cell, and
Brain, respectively, from Ramalho-Santos et al.
(Ramalho-Santos et al., 2002).
Gastric SC (3) and Chief Cell (3) indicate Gastric Stem Cell and Chief Cells,
respectively, from Mills et al. (Mills et
al., 2002; Mills et al.,
2003).
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