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Fig. 4. Expression analysis of wild-type and Dprp-null mice.
(A) Northern analysis for Dprp, Plp-j, Plp-b and Mt1
in decidual tissues. Total RNA was isolated from decidual tissues of wild-type
(+/+) and Dprp-null (-/-) mice on day 7.5 of gestation.
Gapdh was used to demonstrate integrity of the RNA and loading
accuracy. (B) PRL superfamily expression patterns were examined in
mouse placentas using the PRL superfamily miniarray assay. cDNAs for all
members of the mouse PRL superfamily were spotted on to nylon membranes. Total
RNA from day 12.5 or day 17.5 placental tissues were used to make probes by
reverse-transcription. Gapdh and salmon sperm DNA were used as
controls. (C-F) Localization of Dprp (C,D) and Plp-j
(E,F) mRNAs in implantation sites of wild-type (+/+; C,E) and
Dprp-null (-/-; D,F) mice on day 7.5 of gestation. Dprp and
Plp-j plasmids were used as templates for the synthesis of
digoxigenin-labeled sense and anti-sense RNA probes. The sense probes did not
demonstrate specific staining (data not shown). The mesometrial region of the
uterus is located at the top of each image. Scale bars: 1 mm.
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