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Figure 1


Fig. 1. Mad, Med and shn are required to repress sal expression in the Drosophila haltere. The proteins visualized are indicated at the bottom left of each panel; in E-P, the mutant gene in clones is indicated at the top of each panel. The absence of GFP expression indicates the position of homozygous mutant clones. (A,B) Wild-type Sal expression in the wing (A) and haltere (B) imaginal discs. Sal is repressed in the haltere pouch (dashed outline). (C,D) Brk is expressed in both the wing (C) and haltere (D) imaginal discs. (E-H) Sal is not expressed in Mad homozygous mutant clones in the wing (E,F, arrowheads), owing, at least in part, to the repression of sal by the upregulation of Brk in clones (G, arrowhead). (H) Merged image of E-G. (I-P) Sal is derepressed in Mad (I-K, arrowheads), Med (M,N, arrowheads), and shn (O,P, arrowheads) mutant clones in the haltere. (L) Brk is expressed in Mad mutant clones but is insufficient to repress sal expression in the haltere. (K) Merged image of I,J. (N) Merged image of M and GFP channel (data not shown). (P) Merged image of O and GFP channel (data not shown).





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