Supplemental Figure S1 (Adobe PDF) -
Fig. S1. Detailed analysis of Barx1 expression in E12.5 wild-type mouse embryos. (A) Whole-embryo (parasagittal) in situ hybridization, showing that Barx1 mRNA is restricted to developing branchial arches (Ba) and the stomach (St) wall. The latter expression encompasses the mesothelial lining of the stomach, spleen and caudal surface of the liver (Lv), but not the outer intestinal (Int) surface (mesentery). (B-D) Immunohistochemical confirmation that Barx1 is expressed along the surface of the liver closest to the stomach (B), but excluded from the mesentery (C,D). (E) Graphical delineation of the rostrocaudal extent of the domain of abdominal Barx1 expression. The anatomic diagram is taken, without modification, from http://connection.lww.com/Products/sadler/images/figurelarge13-14.jpg and the experimentally determined domain of Barx1 expression is marked with a bracket (}).
