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Fig. 2. Severe mucosal abnormalities in the proximal foregut of
Barx1-/- mice. (A,B) Abnormally proximal
expression of Pdx1 (near the squamous lining) and mixing of
H+/K+-ATPase+ and Pdx1+ cells in
the glandular mucosa of Barx1-/- neonatal stomach. The two
panels show staining on adjacent sections. (C) Radial asymmetry of the
most-proximal mucosa, with one surface lined by a flat squamoid epithelium
(seen at the top of the image) and the other (bottom) by a cuboidal epithelium
containing many ciliated cells (arrows). (D,E) PAS (D) and
Muc5ac staining (E) reveal the presence in both surfaces of cuboidal cells
with features of glandular pit cells. (F) Ultastructural confirmation
of numerous apical cilia in cells lining neonatal Barx1-/-
proximal foregut. (G,H) Immunohistochemical evidence for
expression of Sox2, a squamous foregut marker, shown at low (G) and high (H)
magnification. (I,J) Smooth muscle actin staining indicates
smooth muscle differentiation in Barx1-/- stomach (J),
although both signal strength and tissue continuity are reduced in comparison
to littermate controls (I). (K) Graphic representation of
gastrointestinal homeotic and regional anomalies that occur in the absence of
Barx1.
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