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Figure 6


Fig. 6. Correlates of spleen development and Barx1 expression. (A,B) The spleen (*) appears to be specified correctly in E9.5 Barx1-/- mouse embryos (B), as in control littermates (A). St, stomach; Pa, pancreas. (C,D) Differences in size and organ relationships between control (C) and Barx1-/- (D) embryos are evident by E12.5 and increase thereafter. The position of the mutant spleen is again highlighted with a dotted line in D. (E-G) Radioactive mRNA in situ hybridization at E10.5, E11.5 and E13.5, showing high Barx1 expression in stomach mesenchyme and mesothelium with exclusion from spleen mesenchyme throughout. Bright-field images are shown for the younger embryos and a dark-field image at E13.5. (H,I) Colorimetric mRNA in situ hybridization at E12.5, showing absence of Barx1 expression in intestinal (Int; arrowheads in H) mesentery and presence in the mesothelial lining of the caudal liver (Lv; arrowheads in I), adjacent to the stomach (St). (J,K) Immunohistochemical confirmation with Barx1 (J) and preimmune (K) antiserum of high expression in stomach mesenchyme (top) and mesothelium (arrowheads), with exclusion from the spleen (Sp) mesenchyme in E12.5 embryos.





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