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Fig. 7. Barx1 effects on the spleen are not mediated through either Wnt
signaling or a group of homeodomain transcription factors implicated in
control of spleen development. (A-C) Lack of ß-gal activity in
the pancreas or spleen of Barx1-/-;TOPGAL E18.5 mouse
embryos, shown in situ (A) to contrast with residual activity in the stomach
(St) and native signal in duodenum (Du). The dotted line in A marks the fused
spleen-pancreas (Pa), where absence of ß-gal activity is further revealed
in B and confirmed by microscopic analysis in C. (D) In contrast to
Barx1, homeobox genes previously implicated in spleen development are
mostly expressed in prospective spleen mesenchyme (Sp) and excluded from the
mesothelium. The left column shows low-magnification images from each in situ
hybridization, and the boxed area of each image is shown at higher
magnification in the middle column, where the splenic capsule is demarcated by
dotted lines. Images in the right-hand column reveal that expression of each
of these homeobox genes is maintained in Barx1-/- spleen,
which is recognized in part by juxtaposition to Pdx1+ pancreatic
tissue (red box).
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