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Fig. 1. Close proximity between the PE and liver bud as shown molecularly by
double in situ hybridization. (A-P) Chick embryos at stage 11
(M,N), stage 12 (I,J), stage 14 (A,B,E,F) and stage 16 (C,D,G,H,K,L,O,P)
stained for expression of a PE marker gene (purple) [Wt1 (A-D),
capsulin (E-H), Tbx18 (I-L) or Cfc1 (M-P)] in conjunction
with for expression of a liver marker gene, Hex (turquoise).
Right-side view (A-C,E-G,I-K,O) and ventral side view (M,N) of the
whole-mount-stained embryos. B,F,J and N are high-magnification images of
boxed regions in A,E,I and M, respectively. (D,H,L,P) Semi-sagittal sections
of whole-mount-stained embryos at stage 16, showing that all PE marker genes
are expressed in mesodermal tissue in direct contact with the
Hex-positive liver bud. PE, proepicardium; LiB, liver bud; h, heart;
im, intermediate mesoderm. Scale bars: 100 µm (A-C,E-G,I-K,M-O) and 20
µm (D,H,L,P).
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