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Fig. 4. Homeodomain, bZIP, TCF-like, Oct, Sox, Smad and Myb-like binding sites
are important for R-module transcriptional activity. (A) Alignment
of the P. lividus R-module with the corresponding S.
purpuratus and L. variegatus sequences. The conserved sites
mutated in the functional analysis are underlined in black and the colored
boxes indicate putative transcription factor binding sites identified using
MatInspector. Arrowheads indicate the limit of the R-module. (B)
Sequence comparisons of predicted binding sites within the R-module with known
consensus sequences for transcription factors. (C) Effects of Smad,
homeodomain, bZIP, TCF-like, Oct and Sox site mutations on the transcriptional
activity of the R-module at the hatched blastula stage. The homeodomain site
mutation data are from a triple mutant, whereas TCF-like, bZIP, Oct and Sox
correspond to single mutations within the R-module. (D) Mutation of a
putative Myb-like site increases the transcriptional activity of the R-module
and R-module Smad(-) constructs. The data are presented as the ratio of
luciferase expression between EpGluc and the wild-type module. (E)
Effect of the Myb-like binding site mutation on the spatial expression of a
GFP reporter driven by the R-module.
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