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Figure 5


Fig. 5. nodal expression in the sea urchin relies early on autoregulation and Univin signaling and requires SoxB1 and TCF function. (Aa-h) Effects of SoxB1 morpholino and dnTcf mRNA on the expression of the endogenous nodal and univin genes. (d,e) Injection of SoxB1 morpholino diminishes SoxB1 protein expression. Anti-SoxB1 in green (arrow). (Ba-t,Ca-j) Effects of the Nodal, Alk4/5/7 and Univin morpholinos on the expression of the endogenous nodal and univin genes. (D) Effects of treatment with SB431542, of microinjection of dnTcf mRNA and of Nodal, SoxB1 and Univin morpholinos on the transcriptional activity of the R-module at the hatched blastula stage. The data are presented as the ratio of luciferase expression between EpGluc and the wild-type module. Two different morpholino oligonucleotides directed against univin and soxB1 were used and gave similar results. (E) QPCR analysis of nodal expression in SB431542-treated or MoUnivin-injected embryos. VEB, very early blastula; EB, early blastula; PHB, pre-hatching blastula.





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