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Fig. 2. Specific immunodetection of the Hh-induced phosphorylated form of Cos2
on Ser572. (A) On western blot analysis, the anti-S572P antibody
specifically detects the Hh-induced phosphorylated form of Cos2 on Ser572.
Notice the absence of signal in the non-Hh-expressing cells and the strong
decrease in the Hh-expressing cells treated with fu RNAi (lane 2 in
right panel). (B) After transfection with cos2 and fu
constructs, S2 cells were treated with okadaic acid (OA) to mimic Hh pathway
activation. Notice that phosphorylated Cos2 was absent when cells were
transfected with Fu-KD. (C) S2 cells were transfected with
cos2-WT-myc, fu-WT-HA and different variants of
smo. Phosphorylated Cos2 on Ser572 was increased in the presence of
the SmoDDD variant but was absent in the presence of SmoAAA or Fu-KD.
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