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Fig. 4. Response to DNA damage in lin-35, dpl-1,
efl-1 and efl-2 mutants. (A) Analysis of
DNA-damage-induced germ cell apoptosis. For the data represented by the black
bars, hermaphrodites were synchronized and irradiated at the L4 stage and germ
cell apoptosis analyzed 24 hours post-irradiation. For the data represented by
the gray bars, hermaphrodites were synchronized at the L4 stage, irradiated 12
hours post the L4 stage, and germ cell apoptosis analyzed 24 hours
post-irradiation. Average numbers of apoptotic germ cells per gonad arm of
four (black bars; n=61-79) or three (gray bars; n=38-54)
independent experiments are shown. Error bars represent standard deviations.
Strains were scored blind. m+z- indicates that
animals analyzed were homozygous mutant progeny of heterozygous animals.
(B) Semi-quantitative egl-1 RT-PCR experiments using cDNAs
isolated from unirradiated (0 Gy) or irradiated (100 Gy) wild-type (+/+),
lin-35(n745) [lin-35(lf)],
dpl-1(n3643) or efl-2(tm2359) animals.
Purified cDNA was used as positive control (+), water as negative control (-).
tbg-1 RT-PCR was performed as the control. Representative experiments
of three [lin-35(n745)] and two
[dpl-1(n3643), efl-2(tm2359)] independent
experiments are shown. (C) Quantitative egl-1 RT-PCR
experiments using cDNAs isolated from unirradiated (0 Gy) or irradiated (100
Gy) wild-type (+/+) or lin-35(n745) gonads. Average relative
mRNA abundances of two or three independent experiments, each performed in
triplicates, are shown. Error bars represent standard deviations.
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