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Figure 4


Fig. 4. Absence of PR restricts {Delta}N89ß-catenin-induced precocious development to ductal tips. Carmine-stained wholemounts show precocious development along the lateral borders and tips of ducts in PR+/-;{Delta}N89ß-catenin glands (A) and terminally restricted precocious development in PR-/-;{Delta}N89ß-catenin glands (B). Indirect immunofluorescence for the N-terminal Myc tag epitope detected by the 9E10 antibody shows that transgene expression in all luminal cells is unaffected by PR genotype (C,D). Scale bars: 0.3 mm in A,B; 100 µm in C,D.





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