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Figure 7


Fig. 7. Northern blot analysis of milk protein RNA levels in 26-week-old virgin female mice. 28S and 18S rRNA subunits visualized by ethidium bromide staining provided loading controls. In addition, K18 (Krt18), a luminal cell marker, was used as a positive control for the integrity of the RNA and epithelial content. Three mice of each genotype were examined as indicated: PR+/- (lanes 2-4), PR+/-;{Delta}N89ß-catenin (lanes 8-10), PR-/-;{Delta}N89ß-catenin (lanes 11-13) and PR-/- (lanes 5-7). RNA from lactating (L, lane 14) and virgin (V, lane 1) mice were used as positive and negative controls, respectively.





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