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Fig. 7. Neurodegeneration in npc2a; npc2b double mutant
Drosophila. (A) Survival data for flies of different
genotypes. All mutant designations refer to homozygous animals. KG05307
indicates the starting strain for generating npc2a mutants. The
x-axis indicates the time in days and the y-axis shows the
percentage of flies surviving. (B) Evidence for apoptotic cell death in
the nervous system of mutant flies. Wild-type brains (upper left) had little
or no TUNEL staining, so there was little normal cell death. A small number of
cells were stained by TUNEL in npc2a mutants (upper right, arrow).
Lower left and, magnified, lower right: npc2a; npc2b double mutants
had far more frequent death of neurons (arrow) and tracheal cells (arrowhead).
(C) The apoptotic cells (labeled by TUNEL) in npc2a; npc2b
double mutants included neurons (labeled by anti-Elav, arrow in the merged
panel) and non-neurons (arrowhead in the merged panel). (D)
Synaptotagmin staining of wild-type and npc2a axon bundles. The
accumulation of Synaptotagmin (arrow) within axon tracts was observed in a
small number of axons in npc2a mutants but not wild type.
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