DEV004432 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Whole-mount in situ hybridization of wild-type embryos shows Dhh expression associated with the yolk sac vasculature at E10.5, but fails to detect any expression at E8.5 in embryo proper or extraembryonic structures.

• Supplemental Figure S2 -

  Fig. S2. Induction of endothelial tube formation by Bmp2, Bmp6 and Bmp7. Allantoic bud explants from wild-type and Foxf1^−/− embryos were cultured in vitro for 24 hours with or without addition of 0.1 ng/ml recombinant Bmp, as indicated. Formation of primitive vascular plexa was visualized by immunostaining for Pecam.

• Supplemental Figure S3 -

  Fig. S3. The occurrence of progenitors of definitive hematopoiesis in the allantois is not affected by inactivation of Foxf1. Allantoic bud explants from wild-type and Foxf1^−/− embryos were cultured for 24 hours and progenitors of definitive hematopoiesis were visualized by immunostaining for CD41.