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Fig. 6. Bmp signaling is essential for blood vessel formation and is a mediator
of the vasculogenic activity of Foxf1. Explants of E8.25 allantoic buds
(A-F) and Smo-/- E9.5 yolk sacs (G,H)
were cultured in vitro for 24 hours followed by staining with Pecam antibody
to visualize the capillary network. (A-C) Allantoic explants from wt mouse
embryos grown in the absence (A) or presence (B,C) of the Bmp antagonist
noggin at 1 (B) or 10 (C) ng/ml. (D-F) Rescue of blood vessel formation in
Foxf1-/- explants by exogenous Bmp4. (D) Normal
vasculogenesis in Foxf1-/+ explant. (E)
Foxf1-/- allantoic cells express Pecam, but fail to form a
distinct vascular plexus. (F) Addition of 0.1 ng/ml Bmp4 to
Foxf1-/- explants restored formation of the capillary
network. (G,H) The yolk sac from an E9.5 Smo-/- embryo was
dissected, cut in half and left- and right-hand sides spread on separate
filters with (H) or without (G) Bmp4 (0.1 ng/ml) in the medium. After 24 hours
in culture, explants were fixed and stained with Pecam antibody.
Low-magnification images (top) show the entire explants and higher
magnification views (bottom) show the formation of a vascular plexus in
response to Bmp4.
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