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Figure 10


Fig. 10. Ectopic Slit is not sufficient to induce dendrogenesis outside the CNS. (A,B) Detergent-free immunocytochemistry with anti-HRP (green) and anti-Slit (purple) in embryo with Slit overexpression in muscle-12 (GAL4M12/UAS-slitWT) at hour 16. Box in A indicates the region shown in B. Even when faced with ectopic Slit, the aCC and other axons in the ISN do not produce ectopic collateral processes (circle). However, the RP5 axon fails to innervate its target, muscle-12 (arrow). Muscle-12 extends Slit-positive myopodia to contact the RP5 axon. Scale bar: 5 µm.





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