|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Slit topography spatiotemporally prefigures dendrogenesis.
(A-D) Detergent-free immunocytochemistry with anti-HRP, which labels
neuronal surface (green), and anti-Slit (purple) in wild-type (+/+) at hours 9
(A), 14 (B) and 17 (C), and in Slit null
(slit2/slit2) embryos at hour 14 (D). Single
segments are shown as projected images. The width of CNS is shown by the
horizontal line beneath with gray bars indicating the width and position of
bilateral neuropils. Boxes outline the region in which Slit density was
quantified. (E,F) Slit concentration topography along the
medial-lateral axis (mean±s.e.m.) at hour 9 (n=13
half-segments; E), and at hours 14 and 17 (n=15 and 13; F). The
slit/slit tissues (n=13 and 14 for hours 9 and 14,
respectively) provide the baselines. Slit densities are normalized to the
midline level in the wild type at hour 9 (E) or 14 (F). The horizontal lines
shows the peak of neuropilar Slit accumulation, and arrows indicate its
center. Scale bar: 5 µm.
|
