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Fig. 6. Slit topography correlates to aCC dendrogenesis in robo/robo
mutant. (A) Detergent-free immunocytochemistry with anti-HRP
(green) and anti-Slit (purple) in robo4/robo4
embryos at hour 14. (B) Slit concentration topography (neuropilar
portion, normalized to the midline level in +/+ at hour 14; see
Fig. 3F) in wild type (+/+)
(n=12) and robo4/robo4 (n=12)
embryos at hour 14. Arrows indicate the center of the neuropilar
concentration. (C) Scattergraphs (up to 10 aCCs per genotype) of
dendritic tips in +/+ (n=14) and
robo4/robo4 (n=14) at hour 17. Each
horizontal line indicates an individual aCC neurons and circles indicate the
positions of dendritic tips along its axons. Scale bar: 5 µm.