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Fig. 6. Decreased proliferation and absence of apoptosis of male germ cells in
the PU.1-negative mouse mutant. (Aa-i) BrdU incorporation
was monitored by immunolabeling after 5-hour pulses at E12.5, E13.5 and E15.5
in PU.1G/G homozygotes (b,e,h) and their
PU.1+/+ littermates (a,d,g). (c,f,i) Quantitation by
counts of positive cells in whole-gonad sections (including germ cells and
somatic cells). The faint staining of the mutant cells counted as positive at
E12.5 may reflect either lower rates of replication or repair activity. Note
that the label in the mutant testis at E13.5 and E15.5 is restricted to the
peripheral somatic cells. (Ba-f) Tunel assay for apoptotic cells was
performed on E15.5 (a,b) and E17.5 (d,e) embryos. (c,f) Quantitation of
Tunel-positive cells. Scale bars: 50 µm.
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