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Fig. 7. Arrest of spermatogenesis in the PU.1 mutant gonad.
(Aa-h) Wild-type E18.5 testes (a,c,e,g) and testes of their
PU.1G/G littermates (b,d,f,h) were dissected and grafted
under the tunica albuginea of recipient nude mice. Histological analysis and
PCNA immunostaining were performed 1 week after transplantation. The contorted
shape of the peripheral labeled nuclei in h is a known characteristic of
Sertoli cells. (Ba-d) The same analysis 4 weeks after grafting. At low
magnification, the limit of the graft (upper left) and the host tissue (lower
right) can be seen in a and b, corresponding in b to that between empty
tubules and normal spermatogenesis. Scale bars: 200 µm in Aa,Ab,Ba,Bb; 50
µm in all other panels.
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