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Fig. 1. Identification of cortical granules. (A-C) Succynilated
WGA-labeled cortical granules in C. elegans oocytes (A), and
prometaphase I embryos (B), but not in embryos after meiosis I (C) (DNA in
blue). (D-F) WGA-labeled cortical granules (D) are associated with
SP12::GFP-labeled reticulate ER (E) at the cortex of a metaphase I embryo;
merge in F. (G-I) UGTP-1::GFP-labeled 1 µm vesicles were clustered
in prometaphase I (arrows, G) and redistributed across the cortex by metaphase
I (arrows, H). Smaller cytoplasmic puncta remain after loss of the 1 µm
vesicles (arrows, I). (J-L) WGA staining (J) co-localized with
UGTP-1::GFP (K) in cortical granules in the cortex of a metaphase I embryo;
merge in L. Scale bar: 10 µm.
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