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Fig. 3. A wave of exocytosis during anaphase I. C. elegans embryos
expressing histone::GFP and labeled with the plasma membrane dye FM2-10 were
imaged using SFC every 200 milliseconds. After the chromosome separation
initiated (A), exocytic events (arrows) occurred near the spindle
(B) and spread across the cortex (C,D) before the
completion of anaphase I. Images in C and D are maximum projection summations
of several frames in which a vesicle fusion event occurs. A gap is formed
between the plasma membrane and the vitelline layer near the polar body
(E, bracket). (F) Images of the plasma membrane before and after
exocytosis, labeled with FM2-10 or a fluorescent dextran. Scale bar: 10
µm.
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