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Figure 5


Fig. 5. TEM of APC/C mutant and sep-1(RNAi) embryos. (A) The +2 embryo in a mat-1(ye121) C. elegans did not exit meiosis (asterisk indicates chromosomes in meiotic spindle) and retained cortical granules (arrows). (B) sep-1(RNAi) embryos were not arrested, but retained cortical granules (arrows). (C,D) Comparison of eggshell structures. In wild-type meiosis I embryos that contain cortical granules, a single vitelline layer was present (C). In mitotic embryos, cortical granules were lost and a three-layer eggshell (arrows) structure was observed (D). The +2 embryo in APC/C mutant (E) and sep-1(RNAi) (F) animals contained cortical granules and had a single vitelline layer (arrows), similar to immature wild-type eggshells. Scale bars: 3 µm in A,B; 0.5 µm in C-F.





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