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Fig. 6. The dynamics of the exocytic wave during anaphase I. C.
elegans embryos labeled with histone::GFP and FM2-10 were imaged every
333 milliseconds using MPLSM. (A) Total exocytic events observed in
wild-type, chs-1(RNAi) and cks-1(RNAi) embryos were nearly
twice that of sep-1(e2406) and sep-1(RNAi) embryos.
(B) Kinetic profiles of chromosome separation (plotted as a line) and
exocytic events (columns) from single embryo recordings representative of
average dynamics. Images from the movies are shown in C-K; brackets
indicate distance measured between chromosomes, arrows indicate quantitated
exocytic events. In wild type (green), degranulation initiated after
chromosomes separated by 1.5 µm, and completed before polar body extrusion.
In sep-1(RNAi) (red), chromosome separation was severely reduced, the
wave of exocytosis was reduced and took much longer. By contrast, in
sep-1(e2406) embryos (blue) chromosomes initially separated normally,
but still had a reduced level of exocytosis that began after chromosomes
separated by 2.5 µm. Scale bar: 10 µm.
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