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Fig. 7. Separase localizes to cortical granules in C. elegans. (A) Before ovulation, cytoplasmic GFP::SEP-1 rapidly accumulated in the nucleus, on chromosomes (asterisks) and cortical filaments (arrows). By metaphase I, GFP::SEP-1 was lost from filaments and appeared on cortical granules (B,C, arrows). GFP::SEP-1 disappeared during the exocytic wave in anaphase I and accumulated on the cortex near the polar body (D, arrow). (E,F) During mitosis, SEP-1::GFP localized to centrosomes (arrows), chromosomes (asterisks) and a diffuse cloud around the spindle. (G-I) Immunofluorescence with ${alpha}$ -SEP-1 (green) and DNA (blue). (G) Separase-labeled vesicles (arrows) were absent from the vicinity of the spindle in an embryo that had partly completed the exocytic wave during anaphase I (maximum projection image). Separase localizes to six central spindle elements (arrowhead) between homologous chromosomes and accumulates on the cortex near the polar body (asterisk indicates sperm pronucleus). (H) During prometaphase I, separase localized to filaments in the cortex (arrows) and in the spindle (asterisk). (I) Separase appeared on centrosomes (arrow) and chromosomes (asterisk) during mitosis. Scale bar: 10 µm.

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