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Fig. 6. Spatial relationship between PIN1, PID and MAB4/ENP in cultured
Arabidopsis cells. (A,C) PIN1-GFP was expressed
under the control of its own promoter in Arabidopsis protoplasts (A).
GFP-PID was expressed under the control of the CaMV 35S promoter in
Arabidopsis protoplasts (C). GFP fluorescence images (left) and
Nomarski image (right) were taken with an epifluorescence microscope.
(B,D) Localization of PIN1-GFP (B), GFP-PID (D) (green; left)
and mRFP-MAB4/ENP (red; middle). mRFP-MAB4/ENP and PIN1-GFP or GFP-PID were
co-introduced into Arabidopsis protoplasts. Merged image (right) of
mRFP-MAB4/ENP, and PIN1-GFP (B) and GFP-PID (D). An arrowhead in B indicates
the close proximity of the PIN1-GFP and mRFP-MAB4/ENP florescence, which is
shown enlarged in the inset. Arrows in D indicate the colocalization of
GFP-PID and mRFP-MAB4/ENP florescence, which is shown enlarged in the inset.
Scale bars: 10 µm in A-D.
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