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Fig. 6. Activation of Smad2 signaling in Xenopus neural tissues at
gastrula stages results in inhibition of neural markers. (A)
Schematic representation of the chimeric protein GR-Smad2 and analyses of its
activity in animal caps. GR-Smad2 RNA (0.2 ng) was used. In the absence of
dexamethasone (DEX), GR-Smad2 did not induce mesodermal markers (lane 2).
Activation of GR-Smad2 from blastula to early gastrula stages by DEX (2 µM)
led to mesoderm induction (lanes 3 to 5). However, if activated at mid- to
late gastrula stages (stages 11 to 12), GR-Smad2 no longer induced mesoderm in
animal caps (lanes 6 and 7). (B) Activation of GR-Smad2 in neural
tissues at mid-gastrula stages led to inhibition of Sox2 at different axial
levels along the anteroposterior axis.
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